Part:BBa_K2685007
Detecting parts
This part is our part of the test to detect tetracycline in the environment. Because E. coli is not tolerant to tetracycline, we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration of tetracycline. The ABS value of E. coli was determined by comparing with that of E.coli. The results showed that the highest concentration of tetracycline was 10μg/ml in E.coli and 110μg /ml in E.coli.
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The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.
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Moreover, the tetracycline concentration detected by the detection parts can be as low as 0.005 μg/ml, the detection performance is more stable and the detection accuracy is higher.
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1768
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1622
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1068
Illegal NgoMIV site found at 1228
Illegal NgoMIV site found at 1596 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 69
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